Investigation of in-vitro antioxidant and cytotoxic potential of Crotalaria pallida leaves

This project report is submitted in partial fulfilment of the requirements for the degree of Bachelor of Pharmacy, 2017.

Détails bibliographiques
Auteur principal:	Probha, Nafisa Shama
Autres auteurs:	Akter, Raushanara
Format:	Project report
Langue:	English
Publié:	BRAC University 2018
Sujets:	In-vitro antioxidant Crotalaria pallida leaves Antioxidants Medical plants
Accès en ligne:	http://hdl.handle.net/10361/10729

id	10361-10729
record_format	dspace
spelling	10361-107292019-09-30T05:14:09Z Investigation of in-vitro antioxidant and cytotoxic potential of Crotalaria pallida leaves Probha, Nafisa Shama Akter, Raushanara Department of Pharmacy, BRAC University In-vitro antioxidant Crotalaria pallida leaves Antioxidants Medical plants This project report is submitted in partial fulfilment of the requirements for the degree of Bachelor of Pharmacy, 2017. Catalogued from PDF version of project report. Includes bibliographical references (page 83-86). Crotalaria pallida (C. pallida) is a locally known as Kudug Jhunjhuni, and it is traditionally used for treating urinary problems, swelling of joints, the extract of the leaves is used as vermifuge. Since the antioxidant potential using methanolic leaf extract and cytotoxic activity on HeLa cell line have not been investigated before with C. pallida leaves, this study was aimed to screen these activities. In this study, the phytochemical screening of methanolic leaf extract of C. pallida showed the presence of flavonoids, phenolic compounds, alkaloids, tannins, phytosterols and carbohydrates. Various in-vitro antioxidant studies were performed to determine its antioxidant potential. In DPPH free radical scavenging assay, the IC50 value for C. pallida and reference standard ascorbic acid was found to be 157.01μg/mL and 276.6μg/mL respectively, which infers that, C. pallida has higher free radical scavenging power than the standard ascorbic acid. Whilst the total phenolic content was found to be 141.00mg of gallic acid equivalent (GAE)/g, the total flavonoid content was found to be 144.00mg of quercetin equivalent (QE)/g and the total antioxidant capacity was determined to be 90.7mg of ascorbic acid equivalent (AAE)/g of crude extract for a concentration of 1200μg/mL of methanolic extract of C. pallida. Other antioxidant studies conducted previously on the leaf extract with ethanol, ethyl acetate, chloroform, petroleum ether solvent showed that free radical scavenging activity of C. pallida reached above 80% with ethanolic extract and 60% with other extracts, whereas the study conducted with methanolic leaf extract showed 96.55% scavenging activity. The cytotoxicity of leaf extract was examined on HeLa cell line that was performed by MTT assay. The study indicated that, with the highest 2.0mg/mL concentration of leaf extract the IC50 value was found to be 1.25mg/mL and that inferred about 90% cancerous cell population was dead; which was the highest percentage of mortality of HeLa cells compared to 1.5, 1.0 and 0.5 mg/mL concentrations of leaf extract. Thus the study inferred that C. pallida leaf extract has strong antioxidant and cytotoxic potential. The presence of flavonoid provides a notion to the possibility of antioxidant activity, whereas the strong cytotoxic property attributes to the presence of tannins. The findings of this study pave the way for using this medicinal plant as antioxidant agent and possible anticancer agent. Further research and tests may confirm its’ possibilities and effectiveness for treating oxidative stress degenerative diseases like Alzheimer’s disease, Parkinson’s disease etc. and in the treatment of cervical cancer in future. Nafisa Shama Probha B. Pharmacy 2018-10-18T06:17:34Z 2018-10-18T06:17:34Z 2017 2017-02 Project report ID 13146020 http://hdl.handle.net/10361/10729 en BRAC University project reports are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 86 pages application/pdf BRAC University
institution	Brac University
collection	Institutional Repository
language	English
topic	In-vitro antioxidant Crotalaria pallida leaves Antioxidants Medical plants
spellingShingle	In-vitro antioxidant Crotalaria pallida leaves Antioxidants Medical plants Probha, Nafisa Shama Investigation of in-vitro antioxidant and cytotoxic potential of Crotalaria pallida leaves
description	This project report is submitted in partial fulfilment of the requirements for the degree of Bachelor of Pharmacy, 2017.
author2	Akter, Raushanara
author_facet	Akter, Raushanara Probha, Nafisa Shama
format	Project report
author	Probha, Nafisa Shama
author_sort	Probha, Nafisa Shama
title	Investigation of in-vitro antioxidant and cytotoxic potential of Crotalaria pallida leaves
title_short	Investigation of in-vitro antioxidant and cytotoxic potential of Crotalaria pallida leaves
title_full	Investigation of in-vitro antioxidant and cytotoxic potential of Crotalaria pallida leaves
title_fullStr	Investigation of in-vitro antioxidant and cytotoxic potential of Crotalaria pallida leaves
title_full_unstemmed	Investigation of in-vitro antioxidant and cytotoxic potential of Crotalaria pallida leaves
title_sort	investigation of in-vitro antioxidant and cytotoxic potential of crotalaria pallida leaves
publisher	BRAC University
publishDate	2018
url	http://hdl.handle.net/10361/10729
work_keys_str_mv	AT probhanafisashama investigationofinvitroantioxidantandcytotoxicpotentialofcrotalariapallidaleaves
_version_	1814308055545806848

Investigation of in-vitro antioxidant and cytotoxic potential of Crotalaria pallida leaves

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