A study on relationship of Plasmids with Azithromycin and Ciprofloxacin Resistance in isolates causing Urinary Tract Infection
This thesis is submitted in partial fulfilment of the requirements for the degree of Bachelor of Science in Microbiology, 2018.
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BRAC Univeristy
2018
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10361-106312019-09-30T03:23:52Z A study on relationship of Plasmids with Azithromycin and Ciprofloxacin Resistance in isolates causing Urinary Tract Infection Zaima, Faizah Islam, Namista Department of Mathematics and Natural Sciences, BRAC University UTI Antibiotic resistance This thesis is submitted in partial fulfilment of the requirements for the degree of Bachelor of Science in Microbiology, 2018. Catalogoed from PDF version of thesis report. Includes bibliographical references. The urinary tract infection is caused by the members of Enterobacteriaceae family. The emergence of multi-drug resistant bacteria is a great challenge and concern worldwide. The aim of this research was to determine the transmission of antibiotic resistance gene. This study included antibiotic susceptibility tests on the isolated organisms using both Kirby Bauer method and agar diffusion method, followed by plasmid extraction by Kado and Liu method and the bands were observed by agarose gel electrophoresis. The selected ten isolates are then treated with different concentrations of curing agents (Ethidium Bromide and SDS). After treatment with the curing agents, the above mentioned procedures were repeated for the cured isolates. Plasmid extraction and gel electrophoresis were repeated after curing treatment to determine the loss of plasmid bands. Following gel electrophoresis (before and after curing treatment), the results indicated that all the 10 isolates have lost plasmid bands. Kirby Bauer disk diffusion method is done to determine if the antibiotic resistance was plasmid mediated or chromosome mediated. The results of antibiogram were compared with the previous antibiogram results to determine whether the sensitivity of the cured isolates has changed or not. The isolates will lose their resistance after curing if that resistance is plasmid mediated, otherwise the resistance is chromosome mediated. After that, the MIC & MBC values were also compared (both before and after curing treatment) to re-confirm the results of antibiogram. All the results found from this research are necessary to design the transformation procedure. Following plasmid curing, transformation was also done to determine whether the plasmid was transferred via transformation. Faizah Zaima B. Science in Microbiology 2018-09-17T06:17:09Z 2018-09-17T06:17:09Z 2018 2018-07 Thesis ID 14126014 http://hdl.handle.net/10361/10631 en BRAC University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. application/pdf BRAC Univeristy |
| institution |
Brac University |
| collection |
Institutional Repository |
| language |
English |
| topic |
UTI Antibiotic resistance |
| spellingShingle |
UTI Antibiotic resistance Zaima, Faizah A study on relationship of Plasmids with Azithromycin and Ciprofloxacin Resistance in isolates causing Urinary Tract Infection |
| description |
This thesis is submitted in partial fulfilment of the requirements for the degree of Bachelor of Science in Microbiology, 2018. |
| author2 |
Islam, Namista |
| author_facet |
Islam, Namista Zaima, Faizah |
| format |
Thesis |
| author |
Zaima, Faizah |
| author_sort |
Zaima, Faizah |
| title |
A study on relationship of Plasmids with Azithromycin and Ciprofloxacin Resistance in isolates causing Urinary Tract Infection |
| title_short |
A study on relationship of Plasmids with Azithromycin and Ciprofloxacin Resistance in isolates causing Urinary Tract Infection |
| title_full |
A study on relationship of Plasmids with Azithromycin and Ciprofloxacin Resistance in isolates causing Urinary Tract Infection |
| title_fullStr |
A study on relationship of Plasmids with Azithromycin and Ciprofloxacin Resistance in isolates causing Urinary Tract Infection |
| title_full_unstemmed |
A study on relationship of Plasmids with Azithromycin and Ciprofloxacin Resistance in isolates causing Urinary Tract Infection |
| title_sort |
study on relationship of plasmids with azithromycin and ciprofloxacin resistance in isolates causing urinary tract infection |
| publisher |
BRAC Univeristy |
| publishDate |
2018 |
| url |
http://hdl.handle.net/10361/10631 |
| work_keys_str_mv |
AT zaimafaizah astudyonrelationshipofplasmidswithazithromycinandciprofloxacinresistanceinisolatescausingurinarytractinfection AT zaimafaizah studyonrelationshipofplasmidswithazithromycinandciprofloxacinresistanceinisolatescausingurinarytractinfection |
| _version_ |
1814307048861466624 |