Isolation of Salmonella spp. from raw meat, elucidation of their antibiotic susceptibility pattern and evaluation of the antimicrobial efficacy of Oregano (Origanum vulgare) and Black Sesame (Sesamum indicum)

This thesis is submitted in partial fulfilment of the requirements for the degree of Bachelor of Science in Biotechnology, 2018.

Dades bibliogràfiques
Autor principal:	Moumi, Sunayna Hossain
Altres autors:	Tabrejee, Shamira
Format:	Thesis
Idioma:	English
Publicat:	BRAC Univeristy 2018
Matèries:	Sesamum indicum
Accés en línia:	http://hdl.handle.net/10361/10131

id	10361-10131
record_format	dspace
spelling	10361-101312019-09-30T04:42:16Z Isolation of Salmonella spp. from raw meat, elucidation of their antibiotic susceptibility pattern and evaluation of the antimicrobial efficacy of Oregano (Origanum vulgare) and Black Sesame (Sesamum indicum) Moumi, Sunayna Hossain Tabrejee, Shamira Department of Mathematics and Natural Sciences, BRAC University Sesamum indicum This thesis is submitted in partial fulfilment of the requirements for the degree of Bachelor of Science in Biotechnology, 2018. Cataloged from PDF version of thesis. Includes bibliographical references (page 52-56). With the increasing consumption of beef all over the world, the prospect of Salmonella spp. as a potential reservoir of antibiotic resistance was investigated. As plants are considered new sources of antimicrobial agents against antibiotic resistant bacteria, the antibacterial activity of Oregano (Origanum vulgare) and Black Sesame (Sesamum indicum) were evaluated against the Salmonella spp. isolates. A total of 14 Salmonella spp. isolates were obtained from 20 raw beef samples collected from different areas of Dhaka city. Antibiotic susceptibility test of these isolates was done using 24 antibiotics belonging to eight groups – Aminoglycoside, Beta–Lactam, Lincosamide, Macrolide, Nitroimidazole, Quinolone, Sulfonamide and Tetracycline. The antibiotic susceptibility test reported that all the isolates were resistant to Oxacillin, Erythromycin, Tetracycline, Clindamycin, Co-Trimoxazole, Metronidazole, Sulfamethoxazole, Norflaxacin, and Azithromycin. The results also revealed that the isolates exhibited resistance to Cephalexin and Ceftriaxone (86%), Levofloxacin and Penicillin (79%), Kanamycin (71%), Ceftazidime (64%), Amikacin (43%) and Ciprofloxacin (7%) respectively. In addition, the results revealed that the isolates were intermediately resistant to Streptomycin (71%), Amoxicillin and Ampicillin (64%), Imipenen (43%), Ceftazidime (36%), Penicillin (21%), Cephalexin and Ceftriazone (14%), and Kanamycin (7%). No isolate was resistant to two antibiotics which were Chloramphenicol and Cefixime. As an alternative, the Methanolic, Ethanolic and Aqueous extracts of Oregano and Sesame respectively were subjected against the isolates to evaluate the antimicrobial activity. Remarkable antimicrobial activity was observed by the Methanolic extract of Oregano with average zone of inhibition of 23.6 mm, followed by the Ethanolic extract of Oregano with average zone of inhibition of 17.6 mm and least by the Aqueous extract of Sesame with average zone of inhibition of 10.6 mm. The Aqueous extract of Oregano and the Methanolic and Ethanolic extracts of Sesame did not show any antibacterial activity. This study showed that the Salmonella spp. isolates are becoming resistant to 17 antibiotics that are commonly used for Salmonella spp. illness – Amoxicillin, Levofloxacin, Cephalexin, Ampicillin, Ciprofloxacin, Ceftazidime, Ceftriazone, Nalidixic Acid, Sulfamethoxazole, Imipenen, Amikacin, Clindamycin, Norflaxacin, Azithromycin, Co-Trimoxazole, and Metronidazole. However, commonly used Cefizime and Chloramphenicol were reported to be effective antibiotics in this study. Lastly, the presence specific components responsible for the efficacy displayed by Oregano could yield in the formulation and research of an effective drug to treat highly antibiotic resistant bacteria. Sunayna Hossain Moumi B. Biotechnology 2018-05-13T05:50:14Z 2018-05-13T05:50:14Z 2018 2018-04 Thesis ID 14136004 http://hdl.handle.net/10361/10131 en BRAC University theses are protected by copyright. They may be viewed from this source for any purpose, but reproduction or distribution in any format is prohibited without written permission. 62 pages application/pdf BRAC Univeristy
institution	Brac University
collection	Institutional Repository
language	English
topic	Sesamum indicum
spellingShingle	Sesamum indicum Moumi, Sunayna Hossain Isolation of Salmonella spp. from raw meat, elucidation of their antibiotic susceptibility pattern and evaluation of the antimicrobial efficacy of Oregano (Origanum vulgare) and Black Sesame (Sesamum indicum)
description	This thesis is submitted in partial fulfilment of the requirements for the degree of Bachelor of Science in Biotechnology, 2018.
author2	Tabrejee, Shamira
author_facet	Tabrejee, Shamira Moumi, Sunayna Hossain
format	Thesis
author	Moumi, Sunayna Hossain
author_sort	Moumi, Sunayna Hossain
title	Isolation of Salmonella spp. from raw meat, elucidation of their antibiotic susceptibility pattern and evaluation of the antimicrobial efficacy of Oregano (Origanum vulgare) and Black Sesame (Sesamum indicum)
title_short	Isolation of Salmonella spp. from raw meat, elucidation of their antibiotic susceptibility pattern and evaluation of the antimicrobial efficacy of Oregano (Origanum vulgare) and Black Sesame (Sesamum indicum)
title_full	Isolation of Salmonella spp. from raw meat, elucidation of their antibiotic susceptibility pattern and evaluation of the antimicrobial efficacy of Oregano (Origanum vulgare) and Black Sesame (Sesamum indicum)
title_fullStr	Isolation of Salmonella spp. from raw meat, elucidation of their antibiotic susceptibility pattern and evaluation of the antimicrobial efficacy of Oregano (Origanum vulgare) and Black Sesame (Sesamum indicum)
title_full_unstemmed	Isolation of Salmonella spp. from raw meat, elucidation of their antibiotic susceptibility pattern and evaluation of the antimicrobial efficacy of Oregano (Origanum vulgare) and Black Sesame (Sesamum indicum)
title_sort	isolation of salmonella spp. from raw meat, elucidation of their antibiotic susceptibility pattern and evaluation of the antimicrobial efficacy of oregano (origanum vulgare) and black sesame (sesamum indicum)
publisher	BRAC Univeristy
publishDate	2018
url	http://hdl.handle.net/10361/10131
work_keys_str_mv	AT moumisunaynahossain isolationofsalmonellasppfromrawmeatelucidationoftheirantibioticsusceptibilitypatternandevaluationoftheantimicrobialefficacyoforeganooriganumvulgareandblacksesamesesamumindicum
_version_	1814307336118861824

Isolation of Salmonella spp. from raw meat, elucidation of their antibiotic susceptibility pattern and evaluation of the antimicrobial efficacy of Oregano (Origanum vulgare) and Black Sesame (Sesamum indicum)

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